Mating with seminal vesicle-excised male can affect the uterus phospholipid fatty-acids composition during implantation in an experimental mouse model

Vol. 45 (4): 825-833, July – August, 2019

doi: 10.1590/S1677-5538.IBJU.2018.0485


ORIGINAL ARTICLE

Amir Fattahi 1, 2, Zeinab Latifi 3, Masoud Darabi 3, Ali Salmassi 2, Laya Farzadi 1, Maghsood Shaaker 3, Amir Mehdizadeh 4, Tohid Ghasemnejad 1, Leila Roshangar 5, Mohammad Nouri 1, 2
1 Women’s Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 2 Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 3 Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; 4 Liver and Gastrointestinal Diseases Research Centers, Tabriz University of Medical Sciences, Tabriz, Iran; 5 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

ABSTRACT

Purpose: No comprehensive information is available about uterus fatty acid (FA) change during implantation period and possible effects of the seminal vesicle secretion on it.
Materials and Methods: In this study, we evaluated FA composition of uterus phospholipids during the implantation period in intact and seminal vesicle-excised (SVX) mated female mice. Forty NMRI female mice were divided into control (mated with intact male) and seminal vesicle excised (SVX)-mated (mated with SVX-male) groups.
The phospholipid fatty acids composition was monitored during the fi rst fi ve days of pregnancy using gas chromatography and also implantation rate was evaluated on fi fth day of pregnancy.
Results: We found that levels of linoleic acid (LNA) and arachidonic acid (ARA) showed a decreasing trend from the fi rst to the third day of pregnancy and then started to increase on the fourth day and peaked on the fi fth day. In contrast, the level of saturated FA (SFA) increased on the second and third day of pregnancy compared to the fi rst (p<0.05) and then decreased on the fourth and fi fth. We also found that the seminal vesicle secretion could affect the levels of LNA, ARA, SFA, and PUFA in uterine phospholipids especially on second and third day. Moreover, there was a positive correlation between ARA level and implantation rate in control but not SVX-mated groups.
Conclusions: It can be concluded that several uterus FA that have important roles in early pregnancy could be affected by seminal vesicle secretion.

Keywords: Uterus; Seminal Vesicles; Male

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