Vol. 43 (2): 335-344, March – April, 2017
Salvador Vilar Correia Lima 1, Marcilio Romero Machado 2, Flávia Cristina Morone Pinto 1, Mariana Montenegro de Melo Lira 3, Amanda Vasconcelos de Albuquerque 4, Eugênio Soares Lustosa 2, Jaiurte Gomes Martins da Silva 1, Olávio Campos Jr. 5
1 Núcleo de Cirurgia Experimental, Programa de Pós-Graduação em Cirurgia do Departamento de Cirurgia do Centro de Ciências da Saúde da Universidade Federal de Pernambuco, UFPE, Brasil; 2 Serviço de Urologia do Hospital das Clínicas, Departamento de Cirurgia do Centro de Ciências da Saúde da Universidade Federal de Pernambuco, UFPE, Brasil; 3 Departamento de Patologia, Centro de Ciências da Saúde da Universidade Federal de Pernambuco, UFPE, Brasil; 4 Departamento de Cirurgia do Centro de Ciências da Saúde da Universidade Federal de Pernambuco, UFPE, Brasil; 5 Departamento de Biologia Aplicada à Saúde, Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco, UFPE, Brasil
Objective: To validate the application of the bacterial cellulose (BC) membrane as a protecting bar-rier to the urethra.
Materials and Methods: Forty female Wistar rats (four groups of 10): Group 1 (sham), the urethra was dissected as in previous groups and nothing applied around; Group 2, received a 0.7cm strip of the BC applied around the urethra just below the bladder neck; Group 3, received a silicon strip with the same dimensions as in group 2; Group 4, had a combination of 2 and 3 groups being the silicon strip applied over the cellulosic material. Half of the animals in each group were killed at 4 and 8 months. Bladder and urethra were fixed in formalin for histological analysis.
Results: Inflammatory infiltrates were more intense at 4 months at lymphonodes (80% Grade 2), statistically different in the group 2 compared with groups 1 (p=0.0044) and 3 (p=0.0154). At 8 months, all samples were classified as grade 1 indicating a less intense inflammatory reaction in all groups. In group 2, at 8 months, there was a reduction in epithelial thickness (30±1μm) when com-pared to groups 1 (p=0.0001) and 3 (p<0.0001). Angiogenesis was present in groups 2 and 4 and absent in group 3. In BC implant, at 4 and 8 months, it was significant when comparing groups 4 with 1 (p=0.0159).
Conclusion: BC membrane was well integrated to the urethral wall promoting tissue remodeling and strengthening based on morphometric and histological results and may be a future option to prevent urethral damage.
Keywords: Urinary Incontinence; Urethra; ethyl-2-hydroxyethylcellulose [Supplementary Concept]; Polysaccharides, Bacterial; Biocompatible Materials