The protective effect of Papaverine and Alprostadil in rat testes after ischemia and reperfusion injury

Vol. 44 (3): 617-622, May – June, 2018

doi: 10.1590/S1677-5538.IBJU.2017.0600


ORIGINAL ARTICLE

Mehmet Ali Karagoz 1,Omer Gokhan Doluoglu 1, Hatice Ünverdi 2, Berkan Resorlu 3, Mehmet Melih Sunay 4, Arif Demirbas 4, Tolga Karakan 1, 4, Arif Aydin 5
1 Department of Urology, Ankara Training and Research Hospital, Ankara, Turkey; 2 Deparment of Pathology Ankara Training Research Hospital, Ankara, Turkey; 3 Department of Urology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale, Turkey; 4 Saglik Bakanligi Ankara Egitim ve Arastirma Hastanesi Ankara, Ankara, Turkey; 5 Department of Urology, Faculty of Medicine, Necmettin Erbakan University Meram, Konya, Turkey

ABSTRACT  

Objective: To investigate the effect of papaverine and alprostadil on testicular torsion-detorsion injury in rats.

Materials and Methods: A total of 40 male Wistar-Albino rats were used in this study. Four hours of right testicular torsion was applied to each group, excluding sham oper­ated group. The torsion-detorsion (T/D), T/D + papaverine and T/D + alprostadil groups received saline, papaverine and alprostadil at the same time as surgical detorsion, respectively. At 14 days after the surgical detorsion, ischaemic changes and the degree of damage were evaluated with Cosentino scoring and the Johnson tubular biopsy score (JTBS).

Results: JTBS was determined as 8.8±2.7 in the Sham group, 5.08±1.9 in the T/ D+papaverine group, 5.29±2.3 in the T/D +alprostadil group and 2.86±1.9 in the TD group. The JTBS was determined to be statistically significantly high in both the T/D + papaverine group and the T/D + alprostadil group compared to the T/D group (p=0.01, p=0.009). In the T/D + papaverine group, 3 (43 %) testes were classified as Cosentino 2, 3 (43%) as Cosentino 3 and 1 (14 %) as Cosentino 4. In the T/D +alprostadil group, 5 (50 %) testes were classified as Cosentino 2, 3 (30 %) as Cosentino 3 and 2 (20%) as Cosentino 4.

Conclusion: The present study indicated that spermatic cord administration of alprosta­dil and papaverine showed a protective effect against ischemia/reperfusion injury after right-side testes torsion and histological changes were decreased after testicular isch­emia reperfusion injury.

Keywords: Papaverine; Alprostadil; Spermatic Cord Torsion

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