Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats

Vol. 43 (x): 2017 May 5.[Ahead of print]

doi: 10.1590/S1677-5538.IBJU.2016.0546


ORIGINAL ARTICLE

Mustafa Tusat 1, Ahmet Mentese 2, Selim Demir 3, Ahmet Alver 4,5, Mustafa Imamoglu 6
1 Department of Pediatric Surgery, Kilis State Hospital, Kilis, Turkey; 2 Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical, University, Trabzon, Turkey; 3 Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, Trabzon, Turkey; 4 Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey; 5 Department of Medical Biochemistry, Faculty of Medicine, Recep Tayyip Erdogan University, Rize, Turkey; 6 Department of Pediatric Surgery, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

 

ABSTRACT

Objective: Testicular torsion (TT) refers to rotation of the testis and twisting of the spermatic cord. TT results in ischemia-reperfusion (I/R) injury involving increased oxidative stress, inflammation and apoptosis, and can even lead to infertility. The aim of this study was to investigate the effect of ozone therapy on testicular damage due to I/R injury in an experi­mental torsion model.

Materials and Methods: 24 male Sprague-Dawley rats were divided into 3 groups; sham-operated, torsion/detorsion (T/D), and T/D+ozone. Ozone (1mg/kg) was injected intraperi­toneally 120 minutes before detorsion and for the following 24h. Blood and tissue samples were collected at the end of 24h. Johnsen score, ischemia modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were determined.

Results: Levels of IMA, TOS, OSI, and histopathological scores increased in the serum/tissue of the rats in the experimental T/D group. Serum IMA, TOS, and OSI levels and tissue histo­pathological scores were lower in the rats treated with ozone compared with the T/D group.

Conclusion: Our study results suggest that ozone therapy may exhibit beneficial effects on both biochemical and histopathological findings. Clinical trials are now necessary to con­firm this.

Keywords:  Ischemia; Oxidative Stress; Spermatic Cord Torsion

[Full Text]


print