Lack of evidence of HPV etiology of prostate cancer following radical surgery and higher frequency of the Arg/Pro genotype in Turkish men with prostate cancer

Vol. 43 (1): 36-46, January – February, 2017

doi: 10.1590/S1677-5538.IBJU.2015.0429


Merve Aydin 1, Aliseydi Bozkurt 2, Aytekin Cikman 1, Baris Gulhan 1, Mehmet Karabakan 2, Aysun Gokce 3, Murat Alper 3, Murat Kara 1

1 Department of Medical Microbiology, Faculty of Medicine, Erzincan University, Erzincan, Turkey; 2 Department of Urology, Erzincan University, Mengucek Gazi Training and Research Hospital, Erzincan, Turkey; 3 Department of Pathology, Dıskapı Training and Research Hospital, Ankara, Turkey


Objectives: The aim of this study was to assess the possible role of HPV in the devel­opment of prostate cancer (PCa) and investigate the distribution of the p53 codon 72 polymorphism in PCa in a Turkish population.

Materials and methods: A total of 96 tissues, which had been obtained using a radical surgery method, formalin-fixed and parafin-embedded, were used in this study. The study group consisted of 60 PCa tissues (open radical prostatectomy) and the control group contained 36 benign prostatic hyperplasia tissues (BPH) (transvesical open pros­tatectomy). The presence of HPV and the p53 codon 72 polymorphism was investigated in both groups using real-time PCR and pyrosequencing.

Results: The results of the real-time PCR showed no HPV DNA in any of the 36 BPH tis­sue samples. HPV-DNA was positive in only 1 of the 60 PCa samples (1.7%). The HPV type of this sample was identified as HPV-57. The distribution of the three genotypes, Arg/Arg, Arg/Pro and Pro/Pro was found to be 45.6, 45.6, and 8.8% in the PCa group and 57.1%, 34.3% and 8.6% in the control group, respectively. Compared with the control group, patients with PCa had a higher frequency of the Arg/Pro genotype and Proline allele (odds ratio (OR)=1.67, 95% confidence interval (CI)=0.68-4.09, p=0.044; OR=1.13, 95% CI=0.76-1.68, p=0.021, respectively).

Conclusions: The results of the study do not support the hyphothesis that prostate cancer is associated with HPV infection but indicated that Proline allele can be a risk factor in the development of PCa in the Turkish population.

Keywords: Papillomaviridae; Prostatic Neoplasms; Tumor Suppressor Protein p53

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