Antioxidant enzyme profile and lipid peroxidation products in semen samples of testicular germ cell tumor patients submitted to orchiectomy

Vol. 43 (4): 644-651 July – August, 2017

doi: 10.1590/S1677-5538.IBJU.2016.0323


Camila Sposito 1, Mariana Camargo 1,Danielle Spinola Tibaldi 1, Valéria Barradas 1, Agnaldo Pereira Cedenho 1, Marcílio Nichi 2, Ricardo Pimenta Bertolla 1, Deborah Montagnini Spaine 1
1 Departamento de Cirurgia, Divisão de Urologia, Setor de Reprodução Humana, Universidade Federal de São Paulo, SP, Brasil; 2 Departamento de Reprodução Animal, Faculdade de Medicina Veterinária, Universidade de São Paulo, SP, Brasil


Purpose: To determine enzymatic antioxidant and lipid peroxidation levels in seminal plasma of patients orchiectomized for testicular tumors.

Materials and Methods: The study included 52 patients: 26 control men and 26 orchi­ectomized patients for testicular tumor, of which 12 men had seminoma tumor and 14 men non-seminoma tumor. After semen analysis performed according to the WHO guidelines, an aliquot of semen was centrifuged and the seminal plasma was collected. Lipid peroxidation was performed by thiobarbituric acid reactive substances(TBARS) assay and antioxidant profile was assessed by analyzing catalase, glutathione per­oxidase (GPx) and superoxide anion (SOD) activities using colorimetric assays with a standard spectrophotometer. Data were tested for normality and compared using one-way ANOVA (p<0.05).

Results: Seminoma and non-seminoma groups presented lower sperm concentration and morphology when compared to control group (p=0.0001). Both study groups (sem­inoma and non-seminoma) presented higher TBARS levels when compared to con­trol group (p=0.0000013). No differences were observed for SOD (p=0.646) andGPx (p=0.328). It was not possible to access the enzymatic activity of catalase in any group.

Conclusion: Patients with testicular tumor present increased semen oxidative stress, but no differences were observed in antioxidant levels, even after orchiectomy. This indicates that most likely an increased generation of oxidative products takes place in these patients.

Keywords: Antioxidants; Oxidative Stress; Testicular Germ Cell Tumor; Orchiectomy; Semen

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